In this research chromoproteins, namely phycoerythrin and phycocyanin in Korean layer are isolated and purified and their characteristics and the contents of amino acid are investigated. Three kinds of layer, Yonpyungdo product, Jindo product, and Kohung product are used in the experiments. The results are as following:
1) 0.68g of phycoerythrin and 0.72g of phycocyanin are isolated from 50g of layer.
2) when observed by microscope (1,000 times amplification), the crystals of phycocrythrin have the shape of small needles and phycocyanin that of platelets.
3) Absorption spectra of purified phycoerythrin shows strong absorption peaks at 546, 526, and 496mμ, and phycocyanin shows strong absorption peaks at 610 and 546mμ. After the phycoerythrin and phycocyanin were kept at 4℃ for 24 and 48 hours. The peaks of phycoerythrin are slightly shifted and those of phycocyanin are decreased.
4) In amino acid analysis, sixteen kinds of amino acids are detected in phycoerythrin and phycocyanin. Amino acids found is the greatest amounts in phycoerythrin are glutamic acid, alanine, glycine and leucine. Essential amino acids are found to exist in phycocyanin in greater amounts than in phycoerythrin.
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